PREPARATION OF TISSUES FOR SEM
- Fix tissue immediately after removal from organism in 2% glutaraldehyde in Millonig's Buffer (MB) for at least overnight. The sample can remain indefinitely in this fixative.
- Rinse tissue in fresh MB 3 x 15 min.
- Sequentially dehydrate speciments with acetone for 10 min in each of the following following:
% acetone (v/v in Type I water)
Go directly from the last acetone exhange to critical point drying.
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